Abstract

Background
To investigate the effects of hypertonic saline on the generation of oxygenderived free radicals in neutrophils, this study was designed. Methods: Human PMNs were isolated from healthy volunteers’peripheral blood by the modified Boyum method. Human PMNs were incubated in 140 mM, 180 mM, 200 mM NaCl with or without fMLP, and then the production of H2O2 was measured with a flow cytometer at 5, 30, 60, 120, and 180 minutes. Isolated PMNs were incubated in 180 mM NaCl before fMLP, after fMLP and also at the same time. H2O2 production was measured by flow cytometry. Results: Hypertonic saline resulted in the suppression of fMLP-mediated H2O2 production in human PMNs. There was no statistical difference in H2O2 production according to the NaCl concentration between 180 mM and 200 mM. fMLP-mediated H2O2 production in human PMNs did not decreased at hypertonic saline infusion after fMLP. Administration of hypertonic saline decrease fMLP-mediated H2O2 production in human PMNs and concentration of 180 mM Na+is effective as well as 200 mM Na+. However, the effects of hypertonic saline is minimal when hypertonic saline was administration after PMNs were stimulated. Conclusions: It is considered that early resuscitation is required in clinically.

• Keywords: fMLP, H2O2, Hypertonic solutions, Neutrophils